Deteksi konsentrasi asam sialat dan GSH plasma pada tikus yang di induksi karsinogenesis dengan N,2-Fluorenilasetamida (FAA)

Abstract

The purpose of this study was to detect the concentration of the sialic acid and GSH on plasma male Wistar rats (Rattus norvegicus L.) undergoing carcinogenesis. Eighteen male Wistar rats were divided into 3 groups (6 of each); one group was used as control; the other 2 groups were induced carcinogenesis by FAA with the doses of 20 and 40 g respectively. Aquabidest (control group) or FAA were administered orally daily for eight weeks. Every two weeks since the study began (week 0, 2, 4, 6, and 8), the blood plasma was taken from each group. At the 4th and 8th week, liver was taken. The following measurements were performed: the concentration of the sialic acid plasma (method of Warren), the GSH plasma (method of Ellman) and the histopathological changes of the liver tissue at the 4th and 8th week. Compared to the control group, group of FAA doses 20 and 40 g showed a decrease of the GSH plasma concentration and an increase of the sialic acid plasma concentration. Group of carcinogenesis-induced rat with FAA dose 40 g showed a significant decrease of the GSH plasma concentration since the 2th week, followed by a significant increase of the sialic acid plasma concentration since the 4th week. Moreover, the histopathological changes of the liver tissues showed evidence of carcinogenesis proceed especially in group of FAA dose 40 g. These results confirm that carcinogenesis could be initiated by a significant decrease of the GSH concentration and a significant increase of the sialic acid concentration. Further studies are required to determine oxidative damages on bimolecular such as DNA and protein in order to verify the higher damages.