Potential endophytic microbes selection for antidiabetic bioactive compounds production

Abstract

Endophytic microbes ability to produce bioactive compounds in common with its host plant is an opportunity to get source material antidiabetic drugs are natural, inexpensive and environmentally. With the aim of investigating the potential of endophytic fungi that have the potential to produce antidiabetic bioactive compounds , a total of 45 endophytic fungi were isolated from 6 species of Indonesian medicinal plants, , i.e., Mahoni (Swietenia mahagoni Jacq.), Sambiloto (Andrographis paniculata Ness.), Kumis kucing (Orthosiphon spicatus BBS), Mengkudu (Morinda citrifolia L.), Sirih merah (Piper crocatum L.) and Sirih hitam (Piper ornatum sp.) has been done. Fungi isolates were fermented in Potato Dextrose Broth medium for 14 days, then extracted with ethylacetate followed by Thin Layer Chromatography (TLC) test. Screening was conducted using α-glucosidase test. The results showed that there are 7 fungi isolates, i.e., A.Ap.3F (98.84 and 81.40%), A.Ap.4F (96.87 and 81.40%), B.Ap.1F (98.48 and 87.49%)., B.Os.1F (93.91 and 89.01%), A.Pc.1F (87.66 and 82.08%), B.Pc.1F (85.63 and 89.35%) and B.Pc.2F (83.51 and 87.57%) which gave inhibitory activity to α-glucosidase enzyme. These results demonstrate that 7 endophytic fungi has the potential to produce antidiabetic agents.