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DC Field | Value | Language |
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dc.contributor.author | Abinawanto | - |
dc.contributor.author | Rahayu, S. | - |
dc.contributor.author | Lestari, Retno | - |
dc.date.accessioned | 2016-02-26T09:11:50Z | - |
dc.date.available | 2016-02-26T09:11:50Z | - |
dc.date.issued | 2013 | - |
dc.identifier.issn | 1992-6197 | - |
dc.identifier.other | 10.5829/idosi.gv.2013.10.3.72150 | en_US |
dc.identifier.uri | http://paper.sci.ui.ac.id/jspui/handle/2808.28/57 | - |
dc.description.abstract | The aim of this study was to inversitage the effect of egg yolk as a cryoprotectant on spermatozoa quality of Barbonymus gonionotus, twenty four hours after sub-zero freezing. The ejaculates from a total of three males were diluted with the glucose-base extender + 10% methanol + egg yolk. Egg yolk concentration which was used in this study were: 0%, 5%, 7%, 9%, 11%, 13%, 15% and 17%, respectively. Samples were then equilibrated at 4°C for 10 minutes and were freezed at -34°C for 24 hours. Thawing was carried out at 40°C for 30 seconds. Based on Anova test, there were significant effect (P<0.01) of various concentration of egg yolk on post-thaw sperm motility and viability, but not on post-thaw abnormality, compared to control (0% of egg yolk). According to the Tukey test, the concentration of 15% of egg yolk showed significant difference (P<0.01) on post-thaw motility and viability, respectively. Fifteen percent of egg yolk showed the highest post-thaw sperm motility (96.10±3.31) and sperm viability (85.50±3.11), respectively. | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | International Digital Organization for Scientific Information | en_US |
dc.relation.ispartofseries | Volume 10;No. 3 | - |
dc.source | Global Veterinaria 10 (3): 318-321, 2013 | en_US |
dc.source.uri | http://idosi.org/gv/gv10(3)13/12.pdf | en_US |
dc.subject | Barbonymus gonionotus | en_US |
dc.subject | Cryopreservation | en_US |
dc.subject | Egg Yolk | en_US |
dc.subject | Spermatozoa | en_US |
dc.title | Cryopreservation of Java Barb (Barbonymus gonionotus) Using Egg Yolk as a Cryoprotectant | en_US |
dc.type | Article | en_US |
Appears in Collections: | Journal Collection |
Files in This Item:
File | Description | Size | Format | |
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Abinawanto.Veterinaria.2013.pdf | 313,5 kB | Adobe PDF | View/Open Request a copy |
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