Spermatozoa Quality of Goramy Fish, Osphronemus goramy Lacepede, 1801, Twenty Four Hours Post-cryopreservation: The Role of Dimethyl Sulfoxide (DMSO) as a Cryoprotectant

Abstract

The objective of the study was to find the optimum concentration of dimethyl sulfoxide (DMSO) among 0%, 5%, 7%, 10%, 13%, 15%, and 17%, respectively, on sperm quality of Osphronemus goramy Lacepede, 1801, twenty four hours post-cryopreservation. Sperm was collected by handstripping method, and was put on 2ml of cryotube, and was then diluted by combination of DMSO, and 189M extender. The ratio of sperm and diluent was 1 : 4 according to Horton & Otto (1976). Sample (sperm + diluent) was equlibrated at 4°C for 45 minutes, and was vaporated for 10 minutes, and was then frozen in Liquid Nitrogen for 24 hours. Thawing was carried out at 30°C for 30 seconds. According to Tukey test (P>0.05), 13% of DMSO was showed the highest post-thawed sperm motility (68,58%) and sperm viability (63,5%), and also was showed the lowest post-thawed sperm abnormality (29%), respectively