The Effect of Sucrose on Sperm Quality of Osphronemus goramy Two Days Post-cryopreservation

Abstract

The present study was conducted to investigate the effect of variation concentration of sucrose on sperm quality of Osphronemus goramy 48 hours post-cryopreservation. Mature males Osphronemus goramy obtained from a private commercial hatchery were brought into laboratory. The ejaculates from a total of three males were collected by hand stripping. The ejaculated semen was diluted with the extender (sucrose-fish ringer + 10% methanol; 1:9). The concentrations of sucrose tested were: 0%, 0.1%, 0.3%, 0.5%, 0.7%, and 0.9%, respectively. Samples were then equilibrated at 4 °C for 10 minutes, and were frozen at -34 °C for 48 hours. Thawing was carried out at 40 °C for 1-2 min. Based on ANOVA test, there were significant effect (P < 0.05) of various concentration of sucrose on post-thaw sperm motility, viability, and abnormality, compared to control (0% of sucrose). According to the Tukey test (P < 0.01), the concentration of 0.5% of sucrose showed the highest post-thaw sperm motility (81.62 ± 4.19)%, and post-thaw sperm viability (82.17 ± 2.56)%, and the lowest post-thaw abnormality (12.5 ± 1.52)%. Our finding showed the optimum concentration of sucrose in extender was 0.5% which showed 81.62% post-thaw motility.