The effect of glucose in various concentrations on sperm quality of Barbonymus gonionotus (Bleeker, 1850) 24 hours postcryopreservation

Abstract

In the present study we developed a method of sperm cryopreservation for Java barb Barbonymus gonionotus Bleeker (1850). Sperm was collected by hand-stripping method, and was diluted by combination of glucose (0%, 2%, 4%, 6%, 8%, and 10%), 10% of DMSO, and Kurokura solution. The ratio of milt and diluent was 1 : 3, then equlibrated at 4°C for 45 minutes and was freezed at -34°C for 24 hours. Thawing was carried out at 30°C for 30 seconds. The percentage of fresh sperm motility, viability, and abnormality were 87.68%, 77.25%, and 24.25%, respectively. There were significant effect (P>0.05) of various concentration of glucose on post-thawed sperm viability and abnormality compared to control (0% of glucose). The concentration of 6% glucose showed either the highest post-thawed sperm motility (88.45%) or sperm viability (59.80%), and also showed the lowest post-thawed sperm abnormality (45%).