Please use this identifier to cite or link to this item:
http://paper.sci.ui.ac.id/jspui/handle/2808.28/72
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Abinawanto | - |
dc.contributor.author | Fadhillah | - |
dc.contributor.author | Lestari, Retno | - |
dc.date.accessioned | 2016-03-08T03:20:01Z | - |
dc.date.available | 2016-03-08T03:20:01Z | - |
dc.date.issued | 2009-08 | - |
dc.identifier.issn | 1348-4400 | - |
dc.identifier.other | http://doi.org/10.14882/jrds.102.0.224.0 | en_US |
dc.identifier.uri | http://paper.sci.ui.ac.id/jspui/handle/2808.28/72 | - |
dc.description.abstract | In the present study we developed a method of sperm cryopreservation for Java barb Barbonymus gonionotus Bleeker (1850). Sperm was collected by hand-stripping method, and was diluted by combination of glucose (0%, 2%, 4%, 6%, 8%, and 10%), 10% of DMSO, and Kurokura solution. The ratio of milt and diluent was 1 : 3, then equlibrated at 4°C for 45 minutes and was freezed at -34°C for 24 hours. Thawing was carried out at 30°C for 30 seconds. The percentage of fresh sperm motility, viability, and abnormality were 87.68%, 77.25%, and 24.25%, respectively. There were significant effect (P>0.05) of various concentration of glucose on post-thawed sperm viability and abnormality compared to control (0% of glucose). The concentration of 6% glucose showed either the highest post-thawed sperm motility (88.45%) or sperm viability (59.80%), and also showed the lowest post-thawed sperm abnormality (45%). | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | Japanese Society of Animal Reproduction | en_US |
dc.relation.ispartofseries | Vol. 55;Suplement | - |
dc.source | Journal of Reproduction and Development (The 102nd Meeting of the Society for Reproduction and Development) Vol. 55 Supplement August 2009 | en_US |
dc.source.uri | https://www.jstage.jst.go.jp/article/jrds/102/0/102_0_224/_article | en_US |
dc.subject | cryosurvival | en_US |
dc.subject | Barbonymus gonionotus Bleeker (1850) spermatozoa | en_US |
dc.subject | glucose | en_US |
dc.subject | DMSO | en_US |
dc.subject | Kurokura | en_US |
dc.title | The effect of glucose in various concentrations on sperm quality of Barbonymus gonionotus (Bleeker, 1850) 24 hours postcryopreservation | en_US |
dc.type | Article | en_US |
Appears in Collections: | Journal Collection |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
Abinawanto.Reproduction.2009.pdf | 1,9 MB | Adobe PDF | View/Open Request a copy |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.