Antimicrobial and Identification of Active compund Curcuma xanthorrhiza Roxb

Abstract

The ethanol 70% extract of Curcuma xanthorrhiza Roxb inhibited the growth of Gram positive bacteria S. aureus and S. mutans in a concentration of 1.0-5.0% (w/v), while B. cereus in a concentration of 2.0-5.0% (w/v). The Minimum Inhibitory Concentration (MIC) of ethanol 70% extract toward S. aureus and S. mutans were 0.1% (w/v), while against B. cereus it showed 2.0% (w/v). Phytochemistry analysis of ethanol 70% extract consists of alkaloid, quinone, and terpenoids. Thin Layer Chromatography (TLC) analysis showed five spots, the third spot effectively inhibited Staphylococcus aureus ATCC 25923, Streptococcus mutans type F (MUI) and Bacillus cereus ATCC 11778 and contain terpenoids. Analysis UV-Vis and Infra Red spectrophotometry showed that it contains phenolic group at absorbance 275.2 nm and have hydroxyl (-OH) and carbonyl (C-O) functional groups. The result of GC-MS indicated that the extract which contains one compound is xanthorrhizol m/z 218.