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Title: Magnetic Enzymatic Platform for Organophosphate Pesticide Detection Using Boron-doped Diamond Electrodes
Authors: Pino, Flavio
Tribidasari, Ivandini A.
Nakata, Kazuya
Fujishima, Akira
Merkoçi, Arben
Einaga, Yasuaki
Issue Date: Oct-2015
Publisher: Japan Society for Analytical Chemistry
Series/Report no.: Volume 31;Number 10
Abstract: A simple and reliable enzymatic system for organophosporus pesticide detection was successfully developed, by exploiting the synergy between the magnetic beads collection capacity and the outstanding electrochemistry property of boron-doped diamond electrodes. The determination of an organophosphate pesticide, chlorpyrifos (CPF), was performed based on the inhibition system of the enzyme acetylcholinesterase bonded to magnetic beads through a biotin-streptavidin complex system. A better sensitivity was found for a system with magnetic beads in the concentration range of 10−9 to 10−5 M. The estimated limits of detection based on IC10 (10% acetylcholinesterase (AChE) inhibition) have been detected and optimized to be 5.7 × 10−10 M CPF. Spiked samples of water of Yokohama (Japan) have been measured to validate the efficiency of the enzymatic system. The results suggested that the use of magnetic beads to immobilize biomolecules or biosensing agents is suitable to maintain the superiority of BDD electrodes.
ISSN: 0910-6340
Appears in Collections:Journal Collection

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