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Title: Development of Immunochromatographic Strip Tests for Selective and Quantitative Detection of Melamine
Authors: Wicaksono, Wiyogo P.
Tribidasari, Ivandini A.
Saepudin, Endang
Einaga, Yasuaki
Keywords: anodic stripping voltammetry
boron-doped diamond
gold nanoparticles
immunochromatographic strip test
Issue Date: Sep-2014
Publisher: DRPM Universitas Indonesia
Series/Report no.: Volume 18;No. 3
Abstract: n immunochromatographic strip test based on the complex reaction of antigen-antibody (melamine antimelamine) was developed for quantitative detection of melamine. Gold nanoparticles (AuNP) were used to form AuNP-labeled antibody, which then acted as a biosensor. Melamine quantification was performed by the determination of AuNP using anodic stripping voltammetry technique with a boron-doped diamond as the working electrode. With sample volume of 100 µL and immunoreaction time of 7 min, the developed immunochromatographic strip test produced a linear calibration curve for melamine concentration range of 0–0.6 mg/L, with detection limit of 0.1 mg/L and RSD of ~5%. Furthermore, negative results were obtained for samples containing cyanuric acid and urea, indicating that the developed immunochromatographic strip test has potential for selective and quantitative detection of melamine.
ISSN: 2339-1995
Appears in Collections:Journal Collection

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