Please use this identifier to cite or link to this item:
http://paper.sci.ui.ac.id/jspui/handle/2808.28/181
Title: | Development of Immunochromatographic Strip Tests for Selective and Quantitative Detection of Melamine |
Authors: | Wicaksono, Wiyogo P. Tribidasari, Ivandini A. Saepudin, Endang Einaga, Yasuaki |
Keywords: | anodic stripping voltammetry boron-doped diamond gold nanoparticles immunochromatographic strip test melamine |
Issue Date: | Sep-2014 |
Publisher: | DRPM Universitas Indonesia |
Series/Report no.: | Volume 18;No. 3 |
Abstract: | n immunochromatographic strip test based on the complex reaction of antigen-antibody (melamine antimelamine) was developed for quantitative detection of melamine. Gold nanoparticles (AuNP) were used to form AuNP-labeled antibody, which then acted as a biosensor. Melamine quantification was performed by the determination of AuNP using anodic stripping voltammetry technique with a boron-doped diamond as the working electrode. With sample volume of 100 µL and immunoreaction time of 7 min, the developed immunochromatographic strip test produced a linear calibration curve for melamine concentration range of 0–0.6 mg/L, with detection limit of 0.1 mg/L and RSD of ~5%. Furthermore, negative results were obtained for samples containing cyanuric acid and urea, indicating that the developed immunochromatographic strip test has potential for selective and quantitative detection of melamine. |
URI: | http://paper.sci.ui.ac.id/jspui/handle/2808.28/181 |
ISSN: | 2339-1995 |
Appears in Collections: | Journal Collection |
Files in This Item:
File | Description | Size | Format | |
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Ivandini.Makara.2014.pdf | 2,06 MB | Adobe PDF | View/Open Request a copy |
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