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http://paper.sci.ui.ac.id/jspui/handle/2808.28/315
Title: | Isolation and Structure Elucidation of Eumitrin A1 from Lichen Usnea blepharea Motyka and Its Cytotoxic Activity |
Authors: | Maulidiyah Cahyana, A. Herry Suwarso, Wahyudi Priyono Nurdin, Muhammad |
Keywords: | Eumitrin A1 Usnea blepharea Motyka Cytotoxic Murine Leukemia P388 Cells |
Issue Date: | 2015 |
Publisher: | Sphinx Knowledge House |
Series/Report no.: | Volume 8;Issue 4 |
Abstract: | The purpose of this research was to isolate and elucidate structure of secondary metabolite compoundand to examine its cytotoxic activity of lichen Usnea blepharea Motyka obtained from Sulawesi – Indonesia. Powder of lichen was extracted using an acetone and continued separation by employing column chromatography (CC) with n-hexane and ethyl acetate solvents in a gradient elutionto produce a pure isolate. The structure was determined by UV-Vis, LC-MS, FTIR, 1H-NMR, 13C-NMR, 2D-NMR (HMBC, HMQC, COSY). The UV-Visspectrophotometer measurement was obtained maximumwavelength of 229 nm and 334 nm. The LC-MS data of this compound showed a molecular weight of680. The1H-NMR, 13C-NMR with DEPT spectrums showedthat this compound has 34 carbon atoms consisting of 2 methyl groups, 2 methoxy groups, 1 methyl group, 6 metin groups, 3 methylene groups, and 20 C quartener atoms. The2D-NMR (HMBC, HMQC,COSY) spectrums of analysis could be inferred that this compound is a Eumitrin A1 with the formula structure C34H32O15. The Eumitrin A1 was examined its cytotoxic activityagainst Murine Leukemia P388 cells with IC504.5 µg/mL (very active). |
URI: | http://paper.sci.ui.ac.id/jspui/handle/2808.28/315 |
ISSN: | 0974-4304 |
Appears in Collections: | Journal Collection |
Files in This Item:
File | Description | Size | Format | |
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A.HerryCahyana.IJOP.2015.pdf | 1,19 MB | Adobe PDF | View/Open Request a copy |
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